Immunome Protein

Solutions：Proteomics

Origin：USA

• the Sengenics Immunome protein array contains more than 1600 full-length human proteins.

• EVERY protein is correctly folded and functional.This ensures that both conformational and linear epitopes are recognised.Hence,results from using the Immunome array are far more likely to be biologically meaningful.

• Every protein on the array is cloned in frame with the BCCP folding marker.Misfolded proteins will not become biotinylated and are washed away.

• Only correctly folded proteins will become biotinylated and immobilized on the array.

• Exceptional consistency
  

 Figure A1 shows the CV% variation of 100 replicate samples run on Immunome slides(red) and protein array "PX"(blue).

 As can be observed the mean CV% for Immunome was 3.9%,and 37% for the protein array PX.

• Excellent signal-to-noise ratio
  

   Figures A2* protein array(PX) and A3*(Immunome) clearly show that the Immunome array has very low background and clearly interpretable biomarkers.

    *Independent validation data courtesy of KTH, Stockholm.。

Iressa is a small molecule lung cancer drug that inhibits EGFR tyrosine kinase activity.The Immunome protein array was used to evaluate the specificity of lressa-mediated kinase

inhibition. The results below clearly show that lressa also inhibits the activity of many other kinases.

A custom Immunome array consisting of various mutated versions of the p53 oncoprotein was probed with a cy-3 labelled GADD45 duplex oligo.

Differential DNA binding activity can clearly be observed,demonstrating once again that proteins on the Immunome array platform are functionally active.