Epizyme Liquid fast

Solutions：Cell Biology

Origin：USA

Epizyme fast sealing fluid

·Only 5 minutes to complete the transfer of the film's closure.

·Non protein blocking solution based on polymer material.

   ——Compared to the traditional to the composition of the protein based closed solution, the reagent can also effectively reduce the possible cross reactivity, reduce background; while reducing the membrane antigen of shielding to different degree enhanced signal detection.

·In addition to conventional use, especially suitable for sheep antibody and protein antibody.

  ——The traditional use of skimmed milk powder and the BSA are inevitable with Niu Yuan in closed fluid IgG, antibody high homology of cattle and sheep, which led to the use of the secondary antibody for detection of antibodies against sheep, two more non-specific resistance and the sealant itself, high background.In addition, casein (the main ingredient of skim milk powder) and BSA protein contains different levels of phosphorylation, protein phosphorylation sites of the sealant itself is likely to compete with antibodies to phosphorylated proteins, and makes the signal to reduce or even drown.Using non-protein sealer, can effectively reduce the goat anti-3. high background, enhancement of phosphorylated protein antibody signals.

·Without biotin, it can be applied to biotin detection system.

Epizyme (original Promoton) fast closed liquid using the sample.

A: can slightly enhance the GAPDH antibody detection signal.

B: can significantly enhance the H3K27me3 antibody detection signal.