The ResPep SLi is a fully automated tabletop peptide synthesizer. Exchangeable modules cover synthesis scales for screenings with hundreds of peptides in parallel, the affordable production of speciality peptides in microcolumns to the large scale synthesis of 100 mg or more of up to 300 µmol of up to eight individual peptides in 2, 5 or 10 ml columns.
Easy to set up and use, the ResPep SLi is equally suited to use in core facilities, biological focused laboratories and synthesis units.
·NEW 8-Column Module: up to 8 peptides at a scale of 10-300 µmol
·Plate and Mini-Column Module: small scale and microscale; up to 96 peptides at 1-10 µmol in synthesis plates or 1-15 µmol in microcolumns (standard configuration)
·SPOT Module: automated SPOT synthesis of peptide arrays on two membranes
·CelluSpots™ Module: synthesis for CelluSpots™ peptide arrays
→NEW 8-Column Module
The new 8-column module allows parallel peptide synthesis on resin in up to eight columns at a scale of up to 300 µmol. The module is supplied as standard with an orbital shaker. The synthesis products can be handled individually during work-up to simplify cleavage and filtration from the resin.
→Plate and Mini-Column Module
In the ResPep SLi base configuration up to 96 peptides are made in a 96 well filter plate filled with synthesis support. Alternatively the microcolumn module can be used to synthesize up to 24 peptides or PNAs at a slightly larger scale. Reagents are held in the reaction wells and are extracted by vacuum applied to the bottom of the reaction module. An 4-channel manifold connected to a solvent pump is used for rapid washing.
A unique method to generate large numbers of different peptides is the SPOT method for synthesis of peptide arrays on cellulose membranes. Peptides are synthesized by repeated deposition of activated amino acids as spots on a specially derivatized filter sheet. This entire process has now been fully automated and can be adapted to the ResPep SLi synthesizer. Up to 1200 positions on freely defined grids can be addressed on the two synthesis membranes. An upgrade kit with hardware and software is available. This procedure is subject to extensive licensing conditions.
CelluSpots™ are arrays of peptide-cellulose-conjugates spotted onto planar surfaces such as glass slides. Peptides are synthesized on individual disks consisting of a modified cellulose support which can be dissolved after synthesis. The solutions of individual peptides covalently linked to macromolecular cellulose are then spotted onto a surface of choice. Each disk is placed in a single well of a holding frame with 384 positions connected to the vacuum device of the ResPep SLi instrument. Up to 768 peptides can be synthesized in parallel on two frames placed in the instrument. An upgrade kit with hardware and software is available. This procedure is subject to extensive licensing conditions.
The Slide Spotting Robot can be used to spot the peptide-cellulose-conjugates onto coated slides.