The InsituPro VSi represents the third generation in the INTAVIS InsituPro product line for fully automated in situ hybridization and immunohistochemistry on whole mounts, vibratome sections, thin sections on slides and cells on coverslips.

Features of the InsituPro VSi:

• One system for both - In situ hybridization and Immunostainings

• Automation of related staining techniques (LacZ/X-Gal, histology)

• Processing of whole-mounts, vibratome-sections, glass slides and coverslips

(modules for different applications can be exchanged within 2-3 minutes)

• Recovery of probes and antibodies

• Low running costs due to the use of standard lab buffers (open system)

• Compact size - fits on a standard lab bench

• Flexible software that can easily be adapted to your special needs

• Proven system that is already used by more than 300 research groups world-wide

• User tested advanced protocols (LNA-based miRNA detection, TSA signal amplification)

• Protocols for a wide range of model organisms are available

The InsituPro VSi automatically performs the following steps of a typical in situ protocol:

• Rehydration of specimens

• Permeation with proteinase K or detergents

• Postfixation

• Prehybridization

• Hybridization with individual probes or antibodies

• Posthybridization washes

• Blocking and antibody incubation

• All washing steps

The InsituPro VSi provides:

• Individual treatment of each slide or whole mount sample (e.g. different probes or antibodies, different parameters for permeation)

• Bottle preheating (for posthybridization buffers)

• Freely selectable temperature for each step

• Two ports for large buffer bottles for easy handling of bulk solutions like PBST or TBST

• Heated lid for specimen rack (optional)

• Cooled rack for individual antibodies or probes (optional)

• Intuitive operating software for easy modification of individual protocols

(running on any Windows PC for external protocol adaptation)


InsituPro VSi - Automated in situ Detection

As specimens and tissues processed vary in size and shape, different instrument configurations are available. Each work area configuration is delivered as a complete kit with thermo plate or incubation tub, specimen tray, accessories and operation software. Changing configurations just takes a few seconds.

Sample rack configurations:

Work area kit for 60 small baskets:Up to 60 baskets with 5 mm diameter, e.g. for Medaka, Drosophila, Xenopus and small vibratome sections
Work area kit for 60 medium baskets:
Up to 60 baskets with 10 mm diameter, e.g. for chicken, mouse and medium-sized vibratome sections
Work area kit for 32 large baskets:
Up to 32 baskets with 14 mm diameter, for large vibratome sections and large specimens
Work area kit for 60 slides:
For 60 standard slides (75 mm x 25 mm x 1.0 mm)
Specimen size:
0.035-14 mm for whole mounts and vibratome sections
Number of buffer vials:
16 (1l, 250 ml, 125 ml, 50ml) incl. 2 heated; cooled buffer positions 2 (50 ml)
Number of individual probes:

up to 60 for the basket work areas, 60 for the slides

Volume per basket/slide 200 µl for small baskets, 700 µl for medium baskets, 1600 µl for large baskets and 200 µl for slides

220/240 Volt or 110/115 Volt, 250 W
Dimensions (W x D x H):
57 x 50 x 69 cm, 22.4 x 19.7 x 27.2 inch

Copyright © 2018 LIFESIS All Rights Reserved.


Copyright © 2018 拜诺生物 All Rights Reserved.