Some cells in the blood and tissues of the little but important, such as stem cells, endothelial cells, circulating tumor cells and residual disease cells.Detection and analysis of these rare cells, is the key to understanding disease processes and development mechanisms.Rare cell detection is not an easy task, however, will suffer the effects of sample quality and other factors.

Flow cytometry of each variety of quantitative analysis of cells, in blood, bone marrow and other powerful tools for detecting rare cells in your organization.Rare cell detection often requires a large number of samples to collect enough data, this time tradition flow cytometry and some daunting, because of the large sample means that the processing time is longer, if you do not want the expense of detection sensitivity, you can't rush to speed up the sample rate.

Sonic focus technology, do not do any unnecessary sacrifices

When the sample solution injected into the flow cytometry, cell is randomly distributed in three-dimensional space.Them one by one through a laser beam, is important for accurate data collection.Most of the flow cytometry through the rapid flow of sheath fluid to achieve this.Sheath fluid drive cells and limit it in a central location, and single stream, this process is called hydrodynamic focusing.

Unlike traditional hydrodynamic focusing is, Sonic focus technology using ultrasonic cells clustered in the middle of the sample flow, gathering in a straight line.This ultrasound is greater than 2 MHz, similar to the ultrasound and medical imaging.Acoustic focusing flow cytometry is not under the influence of sample rate, can make cell strong focus on laser measuring points, and sample-independent of the ratio of sheath fluid.

Flux and without sacrificing accuracy

Traditional hydrodynamic focusing technique has a lot of limitations.Flow rate increase will increase the width of the sample flow, cell focusing effect.Farther away the cells deviate from Laser Center, greater the excitation light intensity changes, higher CV values.Researchers often need to analyze flux balance and accuracy.

Sonic focus technology to a large extent with injection velocity has nothing to do, no matter what the proportion of samples and sheath fluid flow, all cells are gathered from laser focus, to avoid fragmentation.So you can capture more photons at high flux guarantees high accuracy analysis of samples.

Acoustic focusing flow cytometry at sampling rates of up to 1 mL/min can also maintain precise focus, allowing researchers to obtain lower CV value, better identification of weak signals and background differences get smaller and better separation.

Rare cell detection speed without sacrificing detection generally need to collect a large number of cells in order to obtain accurate and reliable results.In this case, tradition flow cytometry requires a lot of processing time.However, samples of acoustic focusing flow cytometry operating speed is very fast (up to 1,000 µl), each time you run to collect 20 million cells.Such a high sample rate and sample size helps people quickly and accurately detecting rare cells, does not affect the quality of the data.

Sample preparation without sacrificing precious samples had a greater impact on rare cell detection.For example, the elution and cracking of red blood cell (RBC) can cause significant cell damage and injury.Acoustic focusing flow cytometry support Elution, cleavage-free-flow experiment, can best retain the precious samples, to avoid unnecessary loss of cells, sample preparation steps.

Researchers to be replaced by purposefully diluted sample Elution steps and cracking, fast and effective analysis of low concentration samples such as cerebrospinal fluid (CSF), a small number of stem cells as well as cells of the diluted sample.For the mice's blood, bone marrow and other difficult to collect samples, samples of fine-needle aspiration samples or low yield, researchers can direct dyeing and diluted, no need to wash off or red blood cell lysis.

It is worth mentioning that, Sonic focus processing whole blood samples by flow cytometry can save the sample preparation, no sample loss will not affect the quality of the data.