ITS (Insulin, Transferrin, Selenium) is a chemically defined supplement for a variety of cell culture applications. ITS supplement contains components which are required for optimal performance of serum-free media. Supplementation of ITS to different conventional nutrient media substantially reduces the FBS requirement for routine maintenance of many cell lines.

Components: Contains 1.0 mg/ml insulin from bovine pancreas, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 µg/ml sodium selenite.

Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.