Solutions:Proteomics,Molecular Biology,Pharmaceutical ,Life Science,Purification
Origin:Germany
The DigestPro MSi automates all of the tedious manual steps of in-gel or in-solution digestion protocols:
· reduction of disulfide bonds at 60°C
· alkylation of cysteine residues
· enzymatic digestion at 37°C
· washing of gel slices and elution of the peptides from the gel matrix
In addition, various procedures for subsequent sample preparation can be automated:
· desalting and concentration with Millipore® ZipTips®
· handling of other micro-extraction tips for desalting, enrichment and affinity purification
· use of StageTips
· MALDI target spotting on targets of all manufacturers
· preparation of sandwich spots
· loading of samples on Bruker Anchor plates
The DigestPro MSi offers the following features:
· modular work area setup:
freely adjust the instrument according to your individual needs
· automation of different applications on one platform
· freely selectable temperature for each step
· new cooling option for eluted peptides or in solution digestion
· user friendly software for free adaptation of individual protocols
· use of standard lab buffer (low running costs)
· compact size
Specifications MSi
The DigestPro MSi is operated from a PC with a powerful Windows compatible graphical software. All important parameters are accessible and complex protocols are easily generated from the sample procedures provided. MALDI targets and custom vial racks deviating from the microtiter plate format can be used. All operations can be recorded in a log-file for verification of instrument performance. Protocols and records can be exported to other programs for documentation.
Number of samples per run: | up to 96 |
Operating range: | from low fmol range, depending on MS analysis |
Reagents and buffer vials: | 27 positions (4 cooled) |
Volumes delivered: | down to 0.5 µl |
In-solution module: | different formats available |
Gel volume: | 1mm³ to 30 mm³ |
MALDI target geometry: | any target format adaptable |
Reagent cooling: | four cooled reagent positions |
Heating range in-gel rack: | up to 80°C |
Cooled collection position: | 8°C |
Temp. range in-solution rack: | 8°C to 80°C |
Approximate run time: | Digestion 5 - 1 0 h, MALDI prep 1 - 4 h |
Documentation: | log protocol on PC |
Operation: | PC with graphical software (Windows 7) |
Power: | 220/240 V, 50 Hz or 1 1 0/1 1 5 V, 60 Hz, 250 Watt |
Dimensions: | 57 x 50 x 70 cm (width x depth x height) [22.4 x 1 9.7 x 27.2 inches] |
Weight: | 60 kg |