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Yeast Genomic DNA Purification Kit

Yeast Genomic DNA Purification Kit

Solutions:Proteomics,Immunology,Molecular Biology,Cell Biology

Origin:美国


Simplified Method

Unlike conventional yeast genomic DNA purification methods, this kit contains a non-enzymatic lysis buffer and no glass beads or freeze thawing for cell lysis. It is also phenol, chloroform, and column-free.


Easily Scalable

Kit is optimized for Saccharomyces cerevisiae, with an average yield of 1.5 μg DNA per 5 x 107 cells. May be scaled for large or small samples.


Downstream Compatibility

Kit is compatible with a variety of downstream applications including: PCR, restriction enzyme digestion, and Southern blotting.



Kit Components

·Yeast Lysis Solution

·Ribonuclease A (RNase A), 10 mg/ml Solution

·Protein Precipitation Solution

·TE, pH 8.0

Available in 50 or 200 reaction kit sizes.


AMRESCO’s Yeast Genomic DNA Purification yields more DNA than a competing kit. Genomic DNA was isolated from 8 x 107 Saccharomyces cerevisiae using either AMRESCO’s Yeast Genomic DNA Purification Kit or a competitor’s purification kit. The DNA pellets obtained with both kits were suspended into equal final volumes and then 5 µl and 10 µl volumes were loaded onto an agarose gel for comparison. AMRESCO’s Yeast Genomic DNA Purification Kit samples were observed to have greater signal intensity than the competing kit’s samples due to higher DNA yield. Image courtesy of Dr. Anton Komar, Center for Gene Regulation in Health and Disease, Cleveland State University.



Comparison of PCR amplification using yeast genomic DNA templates. Equal volumes of genomic DNA (gDNA) purified from 5 x 107 Saccharomyces cerevisiae were used as templates in six different PCR reactions designed to produce amplicons from 718 bp to 5,290 bp in length. Results of the amplification were comparable using gDNA isolated with AMRESCO’s Yeast Genomic DNA Purification Kit and a leading competitor’s kit.





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Copyright © 2018 拜诺生物 All Rights Reserved.

苏ICP备09044010号