All-in-One cDNA Synthesis SuperMix

Apparatus

Kits and Reagent

Consumables

All-in-One cDNA Synthesis SuperMix

Solutions：Proteomics,Immunology,Molecular Biology,Cell Biology

Origin：USA

Product advantage

·Good reproducibility: the modified dT Oligo / Primers Random ratio helps to improve qPCR repeatability.

·High compatibility: the cDNA of the reverse transcription product can be applied to the Green SYBR or Probe probe method qPCR.

Product comparison

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Biotool All-in-One cDNA Synthesis SuperMix与Invitrogen SuperScript First-Strand Synthesis System for RT-PCR cDNA Synthetic effect comparison

CDNA synthesis was performed using RNA Total (10 fold gradient) of Hela cells. According to the real-time fluorescence quantitative PCR, using 1 UL of cDNA product was used as template, respectively of high abundance genes actb and moderate demeanor genes B2M and low abundance gene TBP were expression detection and qPCR mix use Biotool it 2x SYBR Green qPCR master mix.

Experimental data show that:

1 All-in-One Biotool cDNA Synthesis SuperMix RNA with different concentration gradient as the template all showed very good linear characteristics and very high amplification specificity.

2. In amplification on the efficiency and detection sensitivity, especially low concentration of RNA as a template. Biotool all in one cDNA synthesis Supermix has Hao is not inferior to the Invitrogen superscript & reg; the performance.

Product description

All-in-One cDNA Synthesis SuperMix Biotool provides a careful optimization of the first strand cDNA synthesis with the reaction solution, which contains an excellent high temperature tolerance of reverse transcriptase. In a tube reaction solution, the pre mixed qRT SuperMix 5x contains all the components needed to reverse transcription from RNA to cDNA, and the cDNA product is suitable for real-time fluorescence quantitative PCR.

Product composition

Component	B24403	B24408
5×qRT SuperMix *	400 μL	1 mL × 2
5 × No RT ControlMix ♥	40 μL	200 μL
RNase-free Water	1 mL × 2	1mL × 10
* contains Buffer, dNTP, Reverse Transcriptase, RNase inhibitor, Oligo dT/Random primer mix. ♥ contains all the components in 5 × qRT SuperMix, except Reverse Transcriptase.

Product application

For real-time fluorescence quantitative PCR, especially low abundance gene or low concentration RNA template, the first strand cDNA synthesis.

Storage condition

-20℃ Storage, shelf life of 1 years. Please try to avoid repeated freezing and thawing, such as frequent use in advance packaging preservation.

Stability test

The experimental data show that compared to storage at - 20 ℃, at a temperature of 4 ℃ storage for 7 days, Biotool all in one cDNA synthesis Supermix whether amplification efficiency or sensitivity are not showed significant difference (the use of the products of reverse transcription cDNA was synthesized, and then Biotool x 2 SYBR Green qPCR master mix of qPCR assays)